Object
Aerobic fast-growing gram negative rods
Parameter
Suseptability testing
Reference standard
Internal method
Internal method identity
B05
Comment
Turbidimetric
Vitek 2
Object
Aerobic fast-growing gram-negative rods
Reference standard
Internal method
Internal method identity
B04
Comment
Biochemical
Vitek 2
Object
Bacteria and yeast colonies
Parameter
Identification of bacteria and yeast
Reference standard
Internal method
Internal method identity
B16
Comment
Mass spectroscopy
MALDI-TOF MS
Parameter
Vancomycin-resistant enterococci
Reference standard
Internal method
Internal method identity
G18
Comment
Real-time PCR
In-house
Light Cycler
Object
Colonies of bacteria
Reference standard
Internal method
Internal method identity
G29
Comment
Real-time PCR
Qualitative
In-house
LightCycler
Object
Bacteria colonies, feces
Parameter
Enterohemoragic E.coli,
Enteropathogenic E.coli,
EHEC-lost shiga toxin (eaeA)
Reference standard
Internal method
Internal method identity
G17
Comment
Real-time PCR Proprietary
Light Cycle
Parameter
Resistance determination
broth dilution
Reference standard
Internal method
Internal method identity
B02
Comment
Broth dilution
SensiTest Colistin
Parameter
Determination of resistance
agardifusion
Reference standard
Internal method
Internal method identity
B01
Parameter
Pathogenic bacteria and fungus
Reference standard
Internal method
Internal method identity
B07
Comment
Culture, evaluation and identification
Bactec FX
Object
Bronchial coolant, pleural fluid, induced sputum, biopsy / autopsy
Parameter
Pneumocyctis jierovecii
Reference standard
Internal method
Internal method identity
G36
Comment
RT-PCR Light Cycler
Object
Expectorate, rinsing fluid, BAL, lung biopsy
Reference standard
Internal method
Internal method identity
G34
Comment
RT-PCR Light Cycler
Parameter
Clostridioides difficile
Reference standard
Internal method
Internal method identity
G26
Comment
Real-time PCR
Qualitiative
Cepheid
GeneXpert
Parameter
Clostridioides difficile
Reference standard
Internal method
Internal method identity
B25
Comment
Membrane enzyme immunoassay test. Combined antigen and toxin detection
Parameter
Shigella, Salmonella, Campylobacter, Yersinia, EHEC, EPEC,EIEC, Vibrio, Aeromonas, and Plesiomonas
Reference standard
Internal method
Internal method identity
B22
Comment
Cultivation and manual assessment / identification
Parameter
Cryptosporidium, Entamoeba histolytica and Giardia lamblia
Reference standard
Internal method
Internal method identity
G28
Comment
Real-time PCR
Qualitative
In-house
Panther Fusion
Parameter
Enterotoxigen E.coli,
Enterohemorrhagic E.coli,
Enteroaggregative E.coli
Reference standard
Internal method
Internal method identity
G10
Comment
Real-time PCR,
In-house developed
Panther Fusion
Parameter
EHEC sub typing
Reference standard
Internal method
Internal method identity
G39
Comment
Real-time PCR
In-house
LightCycler
Object
Feces,
bacterial colonies
Parameter
Yersinia, Campylobacter, Shigella / EIEC, and
Salmonella
Reference standard
Internal method
Internal method identity
G27
Comment
Real-time PCR
Qualitiative
In-house
Panther Fusion
Object
Skin scrape, hair and nails
Reference standard
Internal method
Internal method identity
G32
Comment
Real-time PCR
qualitiative
In-house
LightCycler
Object
Material from genitalia, rectum, newborn, fetus a.s
Parameter
Beta-hemolytic streptococcus group B
Reference standard
Internal method
Internal method identity
B20
Comment
Culture on selective
chromogenic medium,
manual evaluation
Object
Material from skin, soft tissue, knuckles, joints, inner organs, cavities, and foreign bodies
Parameter
Pathogenic bacteria and fungus
Reference standard
Internal method
Internal method identity
B18
Comment
Limit: with the exception of material from the respiratory tract, sinuses, mouth, ear, eye, urogenital mucosa, urine, blood, cerebrospinal fluid and feces
Object
Material from the respiratory tract, sinuses, mouth, ear, and eye
Parameter
Pathogenic bacteria and fungus
Reference standard
Internal method
Internal method identity
B17
Comment
Culture and manual evaluation
Object
Material from the lower respiratory tract
Reference standard
Internal method
Internal method identity
B21
Object
Material from urogenital mucosa
Parameter
Pathogenic bacteria and fungus
Reference standard
Internal method
Internal method identity
B19
Comment
Culture and manual evaluation
Object
Nasopharynx and throat secretion on E-swab
Parameter
Chlamydophila pneumoniae /
Mycoplasma pneumoniae
Bordetella pertussis/
Bordetella parapertussis/
Bordetella holmesii
Reference standard
Internal method
Internal method identity
G02
Comment
Real-time PCR,
In-house
Light Cycler
Panther Fusion
Object
Secretion from rectum, urine, exudate
Parameter
ESBL producing gram-negative rods
Reference standard
Internal method
Internal method identity
B11
Comment
Culture, screening
Object
Secretion from rectum, urine, wounds
Parameter
Vancomycin-resistant enterococci (VRE)
Reference standard
Internal method
Internal method identity
B12
Comment
Culture, screening
Object
Secretion / surface samples and bacterial isolates
Parameter
Meticillin-resistant Staphylococci
Reference standard
Internal method
Internal method identity
G07
Comment
PCR GeneXpert Real-time PCR Light Cycler
Object
Secretion / surface samples and bacterial isolates
Parameter
Methicillin-resistant Staphylococcus aureus (MRSA)
Reference standard
Internal method
Internal method identity
B13
Comment
Culture, screening
Object
Cerebrospinal fluid
Parameter
Pathogenic bacteria and fungus
Reference standard
Internal method
Internal method identity
B08
Comment
Culture, evaluation and identification
Parameter
Legionella antigen
Reference standard
Internal method
Internal method identity
B15
Comment
Immunochromatography membrane test
Binax NOW
Parameter
Urinary tract pathogenic bacteria and fungus
Reference standard
Internal method
Internal method identity
B03
Comment
Culture, evaluation and identification
Parameter
Pneumococcal antigen
Reference standard
Internal method
Internal method identity
B14
Comment
Immunochromatography membrane test
Binax NOW
Object
Urine on dip slide / uricult
Parameter
Urinary tract pathogenic bacteria and fungus
Reference standard
Internal method
Internal method identity
B09
Comment
Culture, evaluation and identification
Object
Urine, secrete from genitalia
Parameter
Neisseria gonorrhoeae
Reference standard
Internal method
Internal method identity
G19
Comment
APTIMA
Qalitative Panther
Object
Urine, secretion from genitalia
Parameter
Mycoplasma genitalium
Reference standard
Internal method
Internal method identity
G14
Comment
APTIMA
Qualitative. Panther
Object
Urine, secretion from genitalia, bacterial colonies
Parameter
Neisseria gonorrhoeae
Reference standard
Internal method
Internal method identity
G20
Comment
Real-time PCR, qualitative
In-house
Light Cycler
Object
Urine, secretion from genitalia
Parameter
Chlamydia trachomatis
Reference standard
Internal method
Internal method identity
G15
Comment
APTIMA
Qualitative, Panther